Sheen showed little emotion and said nothing during his brief arraignment in Pitkin County District Court. The plea was entered by Richard Cummins, one of Sheen's attorneys.
No new details emerged about the Dec. 25 incident.
The star of "Two and a Half Men" on CBS is charged with menacing, criminal mischief and assault. Menacing, the most serious charge, carries a sentence of one to three years in prison.
A judge scheduled a jury trial for July 21.
Sheen is accused of assaulting Brooke Mueller Sheen at an Aspen home while they were on vacation. Charlie Sheen told police they were arguing but denied threatening to kill her.
In court, Sheen wore a black suit, white shirt and black tie. He chatted with a deputy and sipped water from a cup before the hearing started.
Afterward, he walked a few steps to the prosecution table and shook hands with Deputy District Attorney Arnold Mordkin.
Neither Sheen nor his attorneys commented as they left the courtroom.
"Charlie is looking forward to the opportunity to clear his name," said Stan Rosenfield, Sheen's agent.
Brooke Sheen told police her husband threatened her after she told him she wanted a divorce.
Sheen denied threatening his wife with a knife or choking her. He told officers they had slapped each other on the arms and that he had snapped two pairs of her eyeglasses in front of her, according to an arrest warrant affidavit.
Within a week of Sheen's arrest, attorneys for both husband and wife said the couple wanted to reconcile. Sheen's attorney asked the judge to modify a restraining order -- standard in Colorado domestic abuse cases -- that kept them from contacting each other.
On Feb. 8, the judge agreed, and the couple reunited and hugged in an Aspen courtroom.
(Copyright 2010 by The Associated Press. All Rights Reserved.)